List of top Plant Biotechnology Questions on Biochemistry asked in ICAR AIEEA (PG)

Which of the following enzyme finally transfers ubiquitin to the target protein
Match List-I with List-II

List-I (Protein) & List-II (Feature)
(A). $\alpha$-Keratin & (II). Rich in Cysteine residues
(B). Collagen & (III). Rich in Glycine, Proline and Hydroxyproline
(C). Myoglobin & (I). Contains predominantly $\alpha$-helices in its secondary structure
(D). Concanavalin A & (IV). Contains $\beta$-pleated sheets in high abundance

Choose the correct answer from the options given below:
The order of elution (first to last) of amino acids Histidine (H), Glutamic acid (E), and Lysine (K) from a cation exchange column with a buffer having pH 6.0 is (Given: The pK values of H, E, and K is 6.0, 4.25, and 10.5 respectively):
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): Sorghum is a C3 plant.
Reason (R): In Sorghum, the primary $\text{CO}_2$ acceptor is a 3-carbon molecule Phosphoenolpyruvate.
In light of the above statements, choose the correct answer from the options given below.
Amyloids- the insoluble fibrous aggregates deposited in certain tissues- which result in the development of fatal human diseases, can chemically be regarded as:
Match List-I with List-II


List-I (Compound) & List-II (Vitamin D derivative)
(A). Ergocalciferol & (I). Vitamin D2
(B). Cholecalciferol & (II). 25-Hydroxy Vitamin D3
(C). Calcidiol & (III). 1,25-Dihydroxy Vitamin D3
(D). Calcitriol & (IV). Vitamin D3


Choose the correct answer from the options given below:
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).

Assertion (A): During evolution, some species, including humans, became dependent on dietary vitamin C due to loss of their ability to biosynthese ascorbic acid (AA) on their own.
Reason (R): The loss of ability for the biosynthesis of ascorbic acid in human is due to the inactivation of an important enzyme viz. L-Gulono-Lactone Oxidase (GLO).

In light of the above statements, choose the correct answer from the options given below.
In spectrophotometry, if transmittance value of a solution is 75%, then its absorbance would be equal to:
Match List-I with List-II


List-I (Name of the Enzyme) & List-II (Class of the enzyme)
(A). Pyruvate carboxylase & (I). Oxidoreductase
(B). Fumarase & (II). Hydrolase
(C). Phospholipase & (III). Lyase
(D). Nitrogenase & (IV). Ligase


Choose the correct answer from the options given below:
Match List-I with List-II


List-I (Method) & List-II (Use in Protein Chemistry)
(A). Hydrazinolysis & (I). Site-specific cleavage of a polypeptide or protein
(B). Acid/Alkaline Hydrolysis & (II). Amino acid composition analysis of a polypeptide
& or protein
(C). Tryptic Digestion & (III). N-terminal determination of a polypeptide or protein
(D). Sanger Degradation & (IV). C-terminal determination of a polypeptide or protein


Choose the correct answer from the options given below:
The chemical linkage(s) between the $\beta$-D-glucopyranose residues in the structure of $\beta$-glucans isolated from different sources such as cereals, mushroom, bacteria and yeast could be:

(A). Both $\beta$-(1,3) and $\beta$-(1,6)
(B). Both $\beta$-(1,3) and $\beta$-(1,4)
(C). $\beta$-(1,3) only
(D). Both $\beta$-(1,4) and $\beta$-(1,6)

Choose the correct answer from the options given below:
The bicarbonate buffer is a principal buffering system in blood plasma. The ratio of the bicarbonate to carbonic acid required to maintain pH of plasma at 7.4 is:
Which of the following molecules can be classified under glycosphingolipid category?
Match List-I with List-II
Choose the correct answer from the options given below:
"Richmond-Lang effect" on the process of ageing and remobilization of nutrients is associated with this hormone in plants:
Identify the crop plants with "zero" or "nearly zero" $CO_2$ compensation point:
(A). Ragi, Pearl millet, Amaranthus
(B). Cowpeas, Groundnut, Pineapple
(C). Sugarcane, Maize, Foxtail millet
(D). Pineapple, Wheat, Sorghum
Choose the correct answer from the options given below:
Enzymes of HMP shunt pathway are located in:
(A). Cytosol
(B). Plastids
(C). Mitochondria
(D). Peroxisomes
Choose the correct answer from the options given below:
The amino donor to oxoglutarate during photorespiration is:
In plant cells, the principal ion that is electrogenically pumped across membranes in plasmamembrane and tonoplast is:
An example of single membrane cell organelle associated with oil bodies in plant cells is:
The absorbance of a solution of an analyte having 75% transmittance would be equal to (Given log 5= 0.6990 and log 3= 0.4771):
The "Van Deemter equation" describes the elements of band broadening and chromatographic column efficiency in terms of
(A). Eddy diffusion of analyte in the column
(B). Longitudinal diffusion of analyte in the column
(C). Mass transfer of analyte between stationary and mobile phase
(D). Flow rate
Choose the correct answer from the options given below:
The molarity of a buffer soultion made up from a weak acid and its conjugate base would be equal to:
Which of the following patterns is true about the relationship between three pKa values of triprotic phosphoric acid?
Equal volumes of 0.1 M acetic acid and 0.1 M sodium acetate are mixed to form a buffer solution. Considering that the ionization of acetic acid is occuring at dissociation constant of \(1.74 \times 10^{-5}\), what will be its pKa value? (Given: log 1.74 = 0.24)