Question:
Which of the following reagents cannot be used for the determination of the N-terminus of the protein?
Which of the following reagents cannot be used for the determination of the N-terminus of the protein?
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N-terminus reagents:
- Sanger's reagent, Dansyl chloride, Edman's reagent.
- Hydrazine is used for C-terminus determination.
- Sanger's reagent, Dansyl chloride, Edman's reagent.
- Hydrazine is used for C-terminus determination.
Updated On: Sep 1, 2026
- 2,4-Dinitro fluorobenzene
- Dansyl chloride
- Phenylisothiocyanate
- Hydrazine
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The Correct Option is D
Solution and Explanation
Step 1: Understanding the Question:
This question tests knowledge of protein sequencing methods.
Step 2: Detailed Explanation:
Reagents for N-terminus determination:
- 2,4-Dinitro fluorobenzene (A): Sanger's reagent (reacts with free N-terminus).
- Dansyl chloride (B): Reacts with N-terminus to form dansyl derivative.
- Phenylisothiocyanate (C): Edman's reagent (used in Edman degradation).
Hydrazine (D): Used for C-terminus determination, not N-terminus.
It reacts with the C-terminal amino acid to form a hydrazide derivative.
Thus, hydrazine is used for C-terminus, not N-terminus determination.
Step 3: Final Answer:
Thus, hydrazine cannot be used for N-terminus determination, which corresponds to option (D).
[0.5cm]
This question tests knowledge of protein sequencing methods.
Step 2: Detailed Explanation:
Reagents for N-terminus determination:
- 2,4-Dinitro fluorobenzene (A): Sanger's reagent (reacts with free N-terminus).
- Dansyl chloride (B): Reacts with N-terminus to form dansyl derivative.
- Phenylisothiocyanate (C): Edman's reagent (used in Edman degradation).
Hydrazine (D): Used for C-terminus determination, not N-terminus.
It reacts with the C-terminal amino acid to form a hydrazide derivative.
Thus, hydrazine is used for C-terminus, not N-terminus determination.
Step 3: Final Answer:
Thus, hydrazine cannot be used for N-terminus determination, which corresponds to option (D).
[0.5cm]
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