Question:
In Hanes-Woolf Plot, graphing of [S]/v versus [S] yields a straight line whose slope and Y-intercept respectively are:
In Hanes-Woolf Plot, graphing of [S]/v versus [S] yields a straight line whose slope and Y-intercept respectively are:
Show Hint
Hanes-Woolf plot: [S]/v vs [S].
Slope = 1/Vmax, Y-intercept = Km/Vmax.
Other linear plots: Lineweaver-Burk, Eadie-Hofstee.
Slope = 1/Vmax, Y-intercept = Km/Vmax.
Other linear plots: Lineweaver-Burk, Eadie-Hofstee.
Updated On: Sep 1, 2026
- K_m/V_max and 1/V_max
- K_m/V_max and -K_m
- 1/V_max and -1/K_m
- 1/V_max and K_m/V_max
Show Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
Step 1: Understanding the Question:
This question tests knowledge of linear transformations of the Michaelis-Menten equation.
Step 2: Key Formula or Approach:
Michaelis-Menten equation: v = V_max[S]/(K_m + [S])
Rearranging: [S]/v = K_m/V_max + [S]/V_max
This is of the form y = mx + c, where:
y = [S]/v, x = [S],
slope = 1/V_max,
Y-intercept = K_m/V_max.
Step 3: Detailed Explanation:
In Hanes-Woolf plot:
- X-axis: [S]
- Y-axis: [S]/v
- Slope = 1/V_max
- Y-intercept = K_m/V_max
Thus, the slope is 1/V_max and the Y-intercept is K_m/V_max.
Step 4: Final Answer:
Thus, the slope and Y-intercept are 1/V_max and K_m/V_max, which corresponds to option (D).
[0.5cm]
This question tests knowledge of linear transformations of the Michaelis-Menten equation.
Step 2: Key Formula or Approach:
Michaelis-Menten equation: v = V_max[S]/(K_m + [S])
Rearranging: [S]/v = K_m/V_max + [S]/V_max
This is of the form y = mx + c, where:
y = [S]/v, x = [S],
slope = 1/V_max,
Y-intercept = K_m/V_max.
Step 3: Detailed Explanation:
In Hanes-Woolf plot:
- X-axis: [S]
- Y-axis: [S]/v
- Slope = 1/V_max
- Y-intercept = K_m/V_max
Thus, the slope is 1/V_max and the Y-intercept is K_m/V_max.
Step 4: Final Answer:
Thus, the slope and Y-intercept are 1/V_max and K_m/V_max, which corresponds to option (D).
[0.5cm]
Was this answer helpful?
0
0
Top ICAR AIEEA Plant Biotechnology Questions
- Which of the following molecules can be classified under glycosphingolipid category?
- The bicarbonate buffer is a principal buffering system in blood plasma. The ratio of the bicarbonate to carbonic acid required to maintain pH of plasma at 7.4 is:
- Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): The disruption of $\beta$-oxidation of fatty acids in plants can potentially reduce stress-induced ROS accumulation and improve its stress tolerance.
Reason (R): In plants, $\beta$-oxidation of fatty acids takes place predominantly in peroxisomes, where first enzyme of $\beta$-oxidation viz. fatty acyl CoA oxidase produces large amounts of hydrogen peroxide.
In light of the above statements, choose the correct answer from the options given below. - The serum level of which of the following metabolites can effectively distinguish "Vitamin B12 deficiency" from "Folate deficiency" ?
- Match List-I with List-II
List-I (Name of the Enzyme) & List-II (Class of the enzyme)
(A). Pyruvate carboxylase & (I). Oxidoreductase
(B). Fumarase & (II). Hydrolase
(C). Phospholipase & (III). Lyase
(D). Nitrogenase & (IV). Ligase
Choose the correct answer from the options given below:
View More Questions
Top ICAR AIEEA Biochemistry Questions
- Which of the following molecules can be classified under glycosphingolipid category?
- The bicarbonate buffer is a principal buffering system in blood plasma. The ratio of the bicarbonate to carbonic acid required to maintain pH of plasma at 7.4 is:
- Match List-I with List-II
List-I (Name of the Enzyme) & List-II (Class of the enzyme)
(A). Pyruvate carboxylase & (I). Oxidoreductase
(B). Fumarase & (II). Hydrolase
(C). Phospholipase & (III). Lyase
(D). Nitrogenase & (IV). Ligase
Choose the correct answer from the options given below: - Match List-I with List-II
List-I (Method) & List-II (Use in Protein Chemistry)
(A). Hydrazinolysis & (I). Site-specific cleavage of a polypeptide or protein
(B). Acid/Alkaline Hydrolysis & (II). Amino acid composition analysis of a polypeptide
& or protein
(C). Tryptic Digestion & (III). N-terminal determination of a polypeptide or protein
(D). Sanger Degradation & (IV). C-terminal determination of a polypeptide or protein
Choose the correct answer from the options given below: - The chemical linkage(s) between the $\beta$-D-glucopyranose residues in the structure of $\beta$-glucans isolated from different sources such as cereals, mushroom, bacteria and yeast could be:
(A). Both $\beta$-(1,3) and $\beta$-(1,6)
(B). Both $\beta$-(1,3) and $\beta$-(1,4)
(C). $\beta$-(1,3) only
(D). Both $\beta$-(1,4) and $\beta$-(1,6)
Choose the correct answer from the options given below:
View More Questions
Top ICAR AIEEA Questions
- Which of the following statements is false?
- Which of the following is an example of an inventory?
- According to Adam Smith, productivity gains could be brought about through .
- The supply curve for an artificially scarce good is .
- Which of the following would not be considered an opportunity cost of paid employment?
View More Questions