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Food microbiology is the study of microbes that colonise, change, and process food, as well as those that contaminate and spoil it. It is one of the most diverse areas in microbiology research. It includes spoilage, probiotic, fermentative, and pathogenic bacteria, moulds, yeasts, viruses, prions, and parasites, among others. It deals with a wide range of foods and beverages, combining a wide range of environmental conditions that can influence microbial survival and growth.
Process of Food Microbiology
Microorganisms that have favourable or negative impacts on food quality and safety are included in food microbiology and may be of public health importance.
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MCQs Based on Food Microbiology
Ques: In order to make blue cheese, which of the following microbes is used?
- Streptococcus thermophilus
- Lactobacillus bulgaricus
- Penicillium roqueforti
- Rhizopus stolonifer
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Ans: c. Penicillium roqueforti
Explanation: It is necessary to inoculate the curd with the microbe Penicillium roqueforti, which causes the necessary modifications, in order to make blue cheese or Roquefort cheese. Lactic acid bacteria and fungi are primarily responsible for the fermentation and maturing of blue cheeses. Penicillium roqueforti is highly adapted to the low oxygen levels and temperatures experienced during the production of blue cheese.
Ques: Which of the following microorganisms contains a lot of vitamins?
- Bacteria
- Yeast
- Algae
- Protozoa
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Ans: b.Yeast
Explanation: Vitamin content is high in several microorganisms, particularly yeasts. Hydrocarbons mixed with mineral salts make up the yeast cells' growth medium. Nutritional yeast is high in protein, B vitamins, and trace minerals. Many B vitamins are found in it. It's high in thiamine, riboflavin, niacin, vitamin B6, and vitamin B12 when fortified.
Ques: The main microorganism in yoghurt is __________.
- Streptococcus thermophilus
- Leuconostoc citrovorum
- Lactobacillus acidophilus
- Streptococcus lactis
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Ans: a. Streptococcus thermophilus
Explanation: Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus, among other lactic acid bacteria, generate yoghurt, a popular fermented dairy product. These bacteria create lactic acid, which lowers the pH and causes the milk protein to coagulate during the creation of yoghurt. Cultured milk with living bacteria is used to make modern yoghurt. Lactic acid is produced by the bacteria, which causes the milk proteins to coagulate, resulting in thick, sour yoghurt.
Ques: At ______________, the most spoilage bacteria grow.
- acidic pH
- neutral pH
- alkaline pH
- all of the above
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Ans: a. neutral pH
Explanation: The majority of bacteria are neutrophils, which means they thrive at pH levels between 5 and 8, which is one or two pH units below neutral. E. coli, staphylococci, and Salmonella spp. are among the most common bacteria. Fungi, on the other hand, likes pH values of 5.0–6.0, which are somewhat acidic.
Ques: The determination of yeast and mould counts necessitates _________.
- Nutrient agar
- Acidified potato glucose agar
- MacConkey agar
- Violet Red Bile agar
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Ans: b. Acidified potato glucose agar
Explanation: For fungal cultivation, Acidified potato glucose agar is employed. Acidified potato glucose agar is a yeast and mould growth medium that can be treated with acid or antibiotics to prevent bacterial growth. Plate counting procedures for foods, dairy products, and cosmetics testing are all advised. It can be used to produce yeast and moulds that are clinically important.
Ques: Bacterial cells cultured on petroleum industry hydrocarbon wastes are a source of _________.
- carbohydrates
- proteins
- vitamins
- fats
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Ans: d. fats
Explanation: The bacteria can be grown as a protein-rich big cell crop using industrial wastes or by-products as fertilisers. In France, Japan, Taiwan, and India, bacterial cells cultured on hydrocarbon waste from the petroleum industry are used as a source of protein.
Ques: In yeast cells, what is the protein content range?
- 69%
- 12-15%
- 20-40%
- 40-50%
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Ans: d. 40-50%
Explanation: Microbial cells have a very high protein content. Protein content in yeast cells ranges from 40 to 50%. This high-protein component can be used to boost the protein content of food products. Yeast protein is seen as a decent source of protein by 83% of people. Nutritional yeast, often known as nooch, is a deactivated yeast that is offered commercially as a food product. It is usually a strain of Saccharomyces cerevisiae. It's available in the bulk section of most natural food stores in the shape of yellow flakes, granules, or powder.
Ques: When examining coliform bacteria in foods, it is preferable to use______________.
- Mac Conkey broth
- Violet Red Bile agar
- Eosin Methylene blue agar
- All of the above
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Ans: d. All of the above
Explanation: MacConkey Broth, Violet Red Bile agar, and Eosin Methylene blue agar are used to cultivate gramme negative, lactose fermenting bacteria and as a coliform organism presumptive test. The presence of coliform organisms in food, water, milk, and other sanitary materials can be detected using this broth.
Ques: What are the factors that contribute to microbial growth?
- pH
- Moisture
- Oxidation-Reduction Potential
- All of the above
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Ans: d. All of the above
Explanation: These are elements that are present in the food itself. Fish, for example, have properties that may encourage the growth of specific microbes. Intrinsic variables include those that are unique to the food product, such as nutritional content, pH levels, water activity, redox potential, and other antimicrobial components that serve as antimicrobial defence mechanisms.
Ques: Which of the following products is acidic and has no aroma?
- Cultured buttermilk
- Cultured sour cream
- Bulgarian milk
- Acidophilus milk
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Ans: c. Bulgarian milk
Explanation: Sour milk is a favourite among the herds' people all year, although it is more readily available during the summer months when the most milk is produced. Bulgarian milk should not be sweetened or flavoured in any way. The distinctively zingy flavour of natural Bulgarian sour milk, or 'kiselo mlyako,' is due to the region's unique characteristics, including climate, air, and water.
Ques: The ACC (aerobic colony count) is also known as________.
- Total viable count (TVC)
- Aerobic plate count (APC)
- Standard plate count (SPC)
- All of the above
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Ans: d. All of the above
Explanation: Standard plate count, aerobic mesophilic count, total plate count, or aerobic colony count are all terms used to describe the aerobic plate count (APC). The bacterial population in a food sample is estimated using the APC. The total number of bacteria that can develop in an oxygenated or aerobic environment is referred to as the Aerobic Colony Count (ACC). It's one of the most widely used tests to determine the microbiological quality of food, not its safety. Large levels of germs could indicate a lack of cleanliness, as well as issues with process control or ingredient quality. APC is naturally high in some products, such as those made by fermentation.
Ques: Enumeration of microorganisms refers to ______________.
- Depending on the test, non-selective plating may be used.
- Spiral plating, pouring or spreading a food suspension onto suitably selective agar.
- Either A or B
- None of the above
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Ans: c. Either A or B
Explanation: The number of individual live microorganisms in a sample is determined through enumeration in microbiology. Enumerating microorganisms, or determining their numbers, is required to calculate rates of microbial growth and death. It's also common to need to figure out how many germs are in a sample.
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