The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel. The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors. Agarose is extracted from __ .
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel. The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors. Agarose is extracted from __ .
Bacteria
Marine animals
Sea weeds
Fungi
The Correct Option is C
Solution and Explanation
Agarose is extracted from "Sea weeds."
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Concepts Used:
Applications of Biotechnology
Biotechnology is the technology that utilizes biological systems, living organisms, or parts of this to develop or create different products.
For example, brewing and baking bread fall within the concept of biotechnology (use of yeast (= living organism) to produce the desired product).
Agriculture
Alternative to conventional farming: A possible solution is the use of genetically modified crops which is an alternative path to conventional farming.
Medicine
In the area of healthcare, recombinant DNA technological processes have made an immense impact which enables the mass production of safe and more effective therapeutic drugs.
Gene Therapy
A collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo is called Gene Therapy.
CRISPR technology
For editing genomes, CRISPR is a simple yet powerful tool which allows researchers to easily alter DNA sequences and modify gene function.
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