Chromatographic technique/s based on the principle of differential adsorption is/are
A. Column chromatography
B. Thin layer chromatography
C. Paper chromatography
Choose the most appropriate answer from the options given below:
A. Column chromatography
B. Thin layer chromatography
C. Paper chromatography
Choose the most appropriate answer from the options given below:
- B only
- A only
- A & B only
- C only
The Correct Option is C
Approach Solution - 1
Chromatography is a technique used for the separation of components in a mixture based on their different affinities towards a stationary phase while being transported by a mobile phase. Different chromatographic techniques employ different principles for separation, one of which is differential adsorption.
- Understanding Differential Adsorption:
- Differential adsorption is based on the varying degrees to which different components in a mixture adhere to a stationary phase.
- Components that strongly adsorb onto the stationary phase move more slowly compared to those with weaker adsorption, leading to their separation.
- Analysis of Options:
- Column Chromatography (A):
- It utilizes a column packed with a stationary phase (e.g., silica or alumina) and separates compounds based on differential adsorption to the stationary phase.
- Components that have stronger interactions with the stationary phase will travel slowly through the column.
- Thin Layer Chromatography (B):
- Similar to column chromatography, TLC also operates on the principle of differential adsorption.
- A thin layer of adsorbent (typically silica gel) is spread over a glass or plastic plate, and separation occurs based on adsorbent interactions.
- Paper Chromatography (C):
- This technique primarily uses the principle of partition rather than adsorption.
- Separation is based on differences in solubility and paper as a stationary phase is impregnated with a liquid stationary phase.
- Column Chromatography (A):
- Conclusion:
- Column Chromatography and Thin Layer Chromatography both rely on differential adsorption principles.
- Therefore, the correct answer is A & B only.
Approach Solution -2
Chromatographic techniques that are based on the principle of differential adsorption are:
- Column chromatography (A)
- Thin layer chromatography (B)
Both these techniques operate on the principle of differential adsorption, where components of a mixture are adsorbed to different extents on a stationary phase and thus separate during the process.
The Correct Answer is: A & B only
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