Question:
Which of the following is NOT the most commonly used marker gene for plant transformation?
Which of the following is NOT the most commonly used marker gene for plant transformation?
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Marker genes:
- gus, nos, luciferase (reporter genes).
- Selectable markers: nptII, hpt (antibiotic resistance).
- Cry genes are trait genes (not markers).
- gus, nos, luciferase (reporter genes).
- Selectable markers: nptII, hpt (antibiotic resistance).
- Cry genes are trait genes (not markers).
Updated On: Sep 1, 2026
- Nopaline synthase (nos)
- \(\beta\)-glucuronidase (gus)
- Luciferase (lux)
- Cry1Ac gene
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The Correct Option is D
Solution and Explanation
Step 1: Understanding the Question:
This question tests knowledge of marker genes in plant transformation.
Step 2: Detailed Explanation:
Common marker genes for plant transformation include:
- Nopaline synthase (nos): A reporter gene.
- \(\beta\)-glucuronidase (gus): A reporter gene (GUS assay).
- Luciferase (lux): A reporter gene (bioluminescence).
Cry1Ac gene:
- This is an insecticidal gene (Bt gene).
- It is a trait gene, not a marker gene.
- It is used for conferring insect resistance.
Thus, Cry1Ac is not a marker gene.
Step 3: Final Answer:
Thus, Cry1Ac is NOT a marker gene, which corresponds to option (D).
[0.5cm]
This question tests knowledge of marker genes in plant transformation.
Step 2: Detailed Explanation:
Common marker genes for plant transformation include:
- Nopaline synthase (nos): A reporter gene.
- \(\beta\)-glucuronidase (gus): A reporter gene (GUS assay).
- Luciferase (lux): A reporter gene (bioluminescence).
Cry1Ac gene:
- This is an insecticidal gene (Bt gene).
- It is a trait gene, not a marker gene.
- It is used for conferring insect resistance.
Thus, Cry1Ac is not a marker gene.
Step 3: Final Answer:
Thus, Cry1Ac is NOT a marker gene, which corresponds to option (D).
[0.5cm]
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