List of top Plant Biotechnology Questions on Genetic Engineering and Biotechnology

Heat shock method is used to bring about
Which one of the below statements is not true with respect to DNA microarray?
Match List-I with List-II

List-I & List-II
(A). Semiconservative DNA replication & (I). Temin and Baltimore
(B). Reverse transcriptase & (II). Charpentier and Doudna
(C). Gene Editing & (III). Sanger
(D). Insulin & (IV). Watson and Crick

Choose the correct answer from the options given below:
Which of the following is required in CRISPR-Cas9 approach of gene editing
Match List-I with List-II pertaining to scientists and their contribution in various processes/ functioning in plant cells through equations/ laws/ models:
Choose the correct answer from the options given below:
An example for a scorable marker in Genetic engineering
Which of the following cannot be used for confirmation of positive clones?
Which of the following is the activity of alkaline phosphatase?

(1). Addition of 5'-\( \text{PO}_4^{3-} \)
(2). Removal of 5'-\( \text{PO}_4^{3-} \)
(3). Removal of nucleotides from 3'-ends
(4). Removal of single-strand protrusions from the end
Which of the following vector can be used for obtaining single-strand copies of a cloned sequence?

(1). pUC18
(2). Cosmid
(3). \( \lambda \) phage
(4). Phage M13
Which of the following restriction endonuclease requires \( \text{Mg}^{2+} \) for cleavage?

(1). EcoK
(2). EcoRI
(3). EcoB
(4). EcoP1
Non-GM herbicide tolerant rice varieties viz. PB 1979 and PB 1985 have been developed by mutating gene encoding for which one of the following enzymes?
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : The fluorescence of Green Fluorescent Protein (GFP) occurs without the assistance of any helper molecule or prosthetic group.
Reason (R) : Three amino acids-serine, tyrosine and glycine- in the sequence of GFP react between themselves to form a chromophore that imparts light-transducing capability to GFP.
In light of the above statements, choose the correct answer from the options given below:
Which of the following is Type II restriction enzyme?
Which of the following is the activity of reverse transcriptase?
pBluescriptSK is an example of
Which of the following chemical is used to make E. coli cells competent to take external DNA?
Which of the following vector can carry the longest piece of foreign DNA?
Reverse transcriptase enzymes is used in
Type II Restriction Endonucleases are the most important enzymes used for gene cloning. Type-II Restriction endonucleases include
Match List-I with List-II

List-I (Genome edited crops approved for commercial production) & List-II (Trait)
(A) Maize & (I) High level of GABA
(B) Soybean & (II) Non-Browning
(C) Potato & (III) Waxy Starch
(D) Tomato & (IV) Oleic Acid
pBluescriptSK is an example of
Which of the following chemical is used to make E. coli cells competent to take external DNA?
Which of the following is Type II restriction enzyme?
Pressure potential in the xylem of an actively transpiring plant is
In mitochondria, the enzymes of the Krebs Cycle are located