Question:
The enzyme most commonly used to label antibody in immunoassay is:
The enzyme most commonly used to label antibody in immunoassay is:
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Common immunoassay enzymes:
HRP (most common), Alkaline phosphatase, \(\beta\)-galactosidase.
HRP: small, stable, high turnover.
HRP (most common), Alkaline phosphatase, \(\beta\)-galactosidase.
HRP: small, stable, high turnover.
Updated On: Aug 18, 2026
- Alkaline phosphatase
- \(\beta\)-Galactosidase
- Horse radish peroxidase
- Lacto peroxidase
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept:
Immunoassays use enzyme-labeled antibodies.
The choice of enzyme affects sensitivity.
Step 2: Key Formula or Approach:
No specific formula.
Need to know the commonly used enzymes.
Step 3: Detailed Explanation:
Horse radish peroxidase (HRP) is the most commonly used enzyme.
It is used in ELISA and Western blotting.
Alkaline phosphatase and \(\beta\)-galactosidase are also used.
But HRP is the most common.
Lacto peroxidase is not commonly used.
Thus, HRP is the most commonly used enzyme.
Step 4: Final Answer:
Horse radish peroxidase is the most commonly used enzyme.
Hence, the correct option is (C).
Immunoassays use enzyme-labeled antibodies.
The choice of enzyme affects sensitivity.
Step 2: Key Formula or Approach:
No specific formula.
Need to know the commonly used enzymes.
Step 3: Detailed Explanation:
Horse radish peroxidase (HRP) is the most commonly used enzyme.
It is used in ELISA and Western blotting.
Alkaline phosphatase and \(\beta\)-galactosidase are also used.
But HRP is the most common.
Lacto peroxidase is not commonly used.
Thus, HRP is the most commonly used enzyme.
Step 4: Final Answer:
Horse radish peroxidase is the most commonly used enzyme.
Hence, the correct option is (C).
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