List of top Animal Biotechnology Questions on Biotechnology

Which of the following are needed for polymerase chain reaction?
(A). Template DNA segment of interest
(B). Oligonucleotide primers
(C). Taq DNA polymerase
(D). Ribonucleotide triphosphates
Choose the correct answer from the options given below:

Basic function of a fermentor is

Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): Shuttle vector can be propagated in cells of two or more species.
Reason (R): Shuttle vector contain multiple replication of origin and other elements that allows them to be used in more than one species.
Choose the correct answer from the options given below:

Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): Restriction endonuclease is used to cut DNA molecules for cloning.
Reason (R): Restriction endonuclease enzyme cut DNA molecule of any source into unique short pieces in a sequence specific manner.

If a single strand of DNA contain seven restriction sites for a particular restriction endonuclease and none of the restriction site is present either at 3' or 5' end then this enzyme will form how many DNA fragment reacting on it

Which of the following are true for a bacterial artificial chromosome?
(A). It is a kind of artificial plasmid.
(B). It can clone a DNA segment of 100000 to 300000 base pairs.
(C). It can clone a DNA segment of 1000000 to 3000000 base pairs.
(D). It can clone a DNA segment of 3000000 to 5000000 base pairs.
Choose the correct answer from the options given below:

Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): Plasmid can replicate independently but not a DNA fragment separated from a eukaryotic chromosome.
Reason (R): Plasmid contain replication origin.
In light of the above statements, choose the correct answer from the options given below.

Given below are two statements, one is labeled as Assertion (A) and other one labeled as Reason (R).
Assertion (A) : Bacterial artificial chromosome is useful for the synthesis of RNA used for in vitro translation.
Reason (R) : Bacterial artificial chromosome is F factor based cloning vector possessing SP6 and T7 promotor.
In light of the above statements, choose the correct answer from the options given below.

Match List-I with List-II
List-I (Cloning Vector) vs List-II (Characteristic Feature)
(A) Plasmid - (I) Replication restricted to plant gene.
(B) YAC - (II) Replication restricted to mammalian cells.
(C) Cosmid - (III) Replication restricted to bacteria.
(D) Ti Plasmid - (IV) Replication restricted to yeast.
Choose the correct answer from the options given below:

Heat-stable liquids: water, salt solutions, autoclavable media and moderately heat-stable plastics used in animal cell culture can be sterilised by which of the following method?

Which of the following properties best describe holding medium?

In a radioimmunoassay (RIA), the amount of unlabelled antigen is inversely proportional to:

The function of Shine-Dalgarno Sequence is:

Arrange the steps involved in the maintenance of embryonic stem (ES) cell culture in the correct sequence:
A. Prepare feeder plates two days in advance
B. Passaging is done at 1:4 ratio
C. Undifferentiated ES cells require 37° C, 5% CO2, RH 85-100% since they can differentiate spontaneously
D. Trypsinisation or mechanical cutting is done
E. Start feeding 48 hrs after thawing/passaging, every day
Choose the correct answer from the options given below:

Given below are two statements:
Statement I: Brief exposure of washed bovine semen to the saturated solution of EDTA is done to increase IVF results by capacitation
Statement II: Brief exposure of washed bovine semen to high ionic strength media (380 mOsm) or heparin is done to increase IVF result by capacitation
In the light of the above statements, choose the most appropriate answer from the options given below

Choose the correct pair from the options given below

Given below are two statements:
Statement I: Shuttle vectors are used to overexpress proteins ONLY in E. coli.
Statement II: Phagemids contain an origin of replication for phages and bacterial cells
In the light of the above statements, choose the most appropriate answer from the options given below

Given below are two statements:
Statement I: Protein array is powerful proteomic technology using the fusion of microarray and ELISA on protein chip.
Statement II: Protein array is powerful genomic technology using enzyme structure and NMR for limited drug design
In the light of the above statements, choose the most appropriate answer from the options given below

Choose the correct pair from the options given below

Choose the correct pair from the options given below

Among existing technologies , which of the following vector system would you prefer to use for generating a library for 140 kb eukaryotic genomic DNA fragments while giving due consideration to size as well as stability of insert

Given below are two statements:
Statement I: For the culture of mammalian cell lines, glutamine is added to the culture media that serves as the precursor of nucleic acids besides being an important source of energy.
Statement II: Cholesterol and phospholipids are used in the basal media of several serum-free media formulations
In the light of the above statements, choose the most appropriate answer from the options given below