Question:
PCR efficiency is affected by all of the following except:
PCR efficiency is affected by all of the following except:
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PCR efficiency factors:
Primer design, GC content, annealing temperature.
Target sequence length: not a limiting factor.
Primer design, GC content, annealing temperature.
Target sequence length: not a limiting factor.
Updated On: Aug 18, 2026
- Primer length
- Length of target sequence
- Primer sequence
- AT rich sequence
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The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept:
PCR is a technique for DNA amplification.
Various factors affect PCR efficiency.
Step 2: Key Formula or Approach:
No specific formula.
Need to know the factors affecting PCR.
Step 3: Detailed Explanation:
PCR efficiency is affected by:
- Primer length (A)
- Primer sequence (C)
- GC/AT content (D)
The length of the target sequence does NOT generally affect PCR efficiency.
PCR can amplify long or short targets.
Thus, the length of target sequence is the exception.
Step 4: Final Answer:
Length of target sequence does NOT affect PCR efficiency.
Hence, the correct option is (B).
PCR is a technique for DNA amplification.
Various factors affect PCR efficiency.
Step 2: Key Formula or Approach:
No specific formula.
Need to know the factors affecting PCR.
Step 3: Detailed Explanation:
PCR efficiency is affected by:
- Primer length (A)
- Primer sequence (C)
- GC/AT content (D)
The length of the target sequence does NOT generally affect PCR efficiency.
PCR can amplify long or short targets.
Thus, the length of target sequence is the exception.
Step 4: Final Answer:
Length of target sequence does NOT affect PCR efficiency.
Hence, the correct option is (B).
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