List of top Life Science Questions on Biochemistry

Phospholipid vesicles prepared in 50 mM KCl were diluted in water. Based on this information, statements P and Q are made.
P: The diluted vesicles will develop membrane potential.
Q: There is a K+ concentration difference across the vesicular membrane.
Which one of the following options is correct?
 

Which one of the following ion channels opens to repolarize the neuronal membrane when an action potential is generated?
Which of the following is/are peptide hormone(s)?
Peptide-binding cleft in MHC-I is formed by
Which of the following is/are heteropolysaccharide(s)?
Which of the following peptides do/does NOT absorb ultraviolet light above 250 nm wavelength?
The equilibrium dissociation constant of acetic acid is \( 1.74 \times 10^{-5} \) M. The \( pK_a \) of acetic acid (rounded off to one decimal place) is ......
Förster Resonance Energy Transfer does NOT depend on the
Which one of the following is the most sensitive immunoassay?
Which one of the following restriction endonucleases is a blunt cutter?
Which one of the following molecules does NOT contain phosphoanhydride bond(s)?
Which of the following statements about antibodies is/are correct?
Which one of the following molecules captures \(CO_2\) in the \(CO_4\) cycle?
Which one of the following methods separates biomolecules based on their hydrodynamic volumes?
Match the following: A. Polymers – DNA, RNA; B. Polymerase – DNA polymerase, RNA polymerase.
What is the function of Histatin?
Match List I with List II:
List I (Enzyme)List II (Reaction)
(A) DNase I(I) Produces single-stranded nicks in DNA
(B) RNase A(II) Degrades single-stranded RNA
(C) S1 nuclease(III) Degrades single-stranded DNA
(D) RNase H(IV) Cleaves the RNA strand of RNA-DNA hybrids
Choose the correct answer from the options given below:
Of the amino acids listed below, which three amino acids can undergo posttranslational modification?

Match the items in List I with items in List II


 

A mixture of proteins (A, B, C and D) is separated on a Sephadex G-200 column. The proteins elute in the order of A, B, C and D. Assuming that all proteins are globular and monomeric, the protein with minimum electrophoretic mobility on SDS-PAGE will be
In N-linked glycoproteins, carbohydrate moiety is attached to which of the following amino acids?

Match the items in List I with items in List II.
Choose the correct answer from the options given below:

Many enzymes catalyze both forward and reverse reactions. Which one of the following statement is NOT correct?
A biochemist is pelleting down the microsomal fraction from a sample using ultracentrifuge at a speed of 20000 rpm. What would be RCF if the diameter of the rotor is 7 cm?
Which of the following are components of lipid rafts in plasma membrane?
(A) Cholesterol
(B) Sphingolipids
(C) Glycolipids
(D) Phospholipids
Choose the correct answer from the options given below: