List of top Plant Biotechnology Questions on Molecular Biology asked in ICAR AIEEA (PG)

The linking number (L) of a supercoiled duplex DNA can be equated to its Twist (T) and Writhe (W) by which one of the following expression?

Which of the following E. coli DNA polymerase possesses both 5'- and 3'-Exonuclease activity and is thus its own Proofreader and Editor?

Which type of RNA molecules make up more than 80% of the total RNA in the cell?

Majority of the tRNA molecules have the following sequence at their 3'-end:

The symbol "W" in the TWC stem-loop of the tRNA denotes which one of the following?

Given below are two statements:
Statement I: The cap present at the 5'-end of eukaryotic mRNAs is 7-methyl GTP.
Statement II: The 5' cap of eukaryotic mRNAs is essential for their binding by eukaryotic ribosomes and it also enhances their stability.
In the light of the above statements, choose the most appropriate answer from the options given below:

The identification of specific DNA sequence by Southern Blotting involves the following procedures:
A) Digestion of the DNA with a restriction enzyme
B) Fractionation of DNA fragments
C) Transfer of ssDNA molecules from gel to an absorbent sheet
D) Hybridization of radioactively labelled probe to target DNA
What is the correct sequence in which these procedures are executed?