List of top Plant Biotechnology Questions on Molecular Biology asked in ICAR AIEEA (PG)

Match List-I with List-II
{|p{7cm}|p{7cm}|} List-I & List-II
(A) RNA sequencing & (I) Global gene expression of known gene
(B) Northern hybridization & (II) Transcript expression
(C) qPCR & (III) Relative transcript expression
(D) Microarray & (IV) Global gene expression of unknown gene

Match List-I with List-II
{|p{7cm}|p{7cm}|} List-I & List-II
(A) Southern hybridization & (I) Western blotting
(B) Presence of protein of interest & (II) Y-2H
(C) Peptide sequence & (III) Integration of transgene
(D) Interaction of two transcription factors & (IV) Mass spectrometry

Which of the following is true about E. coli DNA ligase
(A) Catalyzes the formation phosphodiester bond between 3'-OH and 5'-$PO_4^-$ group of a DNA strand
(B) Requires ATP as energy source
(C) Requires NAD+ as energy source
(D) It can ligate two molecules of single-stranded DNA
Choose the correct answer from the options given below:
Which of the following statement is correct for 70S ribosome?
(A) The 16S RNA belongs to small subunit.
(B) It also has 8S RNA.
(C) The 5S RNA belongs to 50S subunit.
(D) It can be dissociated into 40S and 30S subunit.
Choose the correct answer from the options given below:
Which of the following is the activity of reverse transcriptase?
In Northern hybridization?
Which of the following subunit of RNA polymerase complex is absent during elongation phase of bacterial transcription?
Which of the following is not related to termination of a transcript in E. coli?
What are isoaccepting tRNAs?
The consensus sequence 5'-ACCAUGG-3'; is also known as
In a nucleotide sequence ATGC, which of the following nucleotide has the unlinked 5' - OH?
Nucleosome- a fundamental unit of chromatin- contains a nucleosome core, which is composed of 146 bp (approx.) of DNA wrapped around histone octamer having which of the following composition?
Identify the correct sequence of \(e^-\) flow leading to ATP synthesis in cyclic-photophosphorylation:
Which of the following is also known as golden fibre?
Bt brinjal was developed for resistance to
Joining two DNA molecules using an adapter involves the following steps
A. Artificial synthesis of an adapter
B. Treating with polynucleotide kinase
C. Blunt end ligation to add an adapter to a blunt-ended DNA molecule
D. Joining two DNA molecules with sticky ends
What is the correct sequence in which these steps are performed?
Match List I with List II
\includegraphics[width=0.45\linewidth]{51.png
Choose the correct answer from the options given below:}
Match List I with List II
\includegraphics[width=0.45\linewidth]{50.png
Choose the correct answer from the options given below: }
Which of the following is correct statement for RNA synthesis?
A. A single RNA polymerase can synthesize all types of RNA in prokaryotes
B. RNA polymerase II can synthesize mRNA in eukaryotes
C. A single RNA polymerase can synthesize all types of RNA in eukaryotes
D. RNA polymerase II can synthesize all types of RNA in prokaryotes
E. E. coli cell has only one type of RNA polymerase
Choose the correct answer from the options given below:
Which of the following technique(s) can be used to study differential gene expression in two tissues under two different external conditions?
A. Northern hybridization
B. Whole genome RNA sequencing
C. qPCR
D. Semi-quantitative PCR
E. Whole Genome Sequencing
Choose the correct answer from the options given below:
The world’s leading exporter of sunflower seed oil is
The amount of carbon dioxide assimilated by photosynthesis divided by amount of water transpired is known as
The precursor for ethylene synthesis in plants is
The principal function of the light-dependent reactions of photosynthesis is to generate
In plants, the tubular extensions of plasma-membrane that connect the cytoplasm of adjacent cells are called