List of top Plant Biotechnology Questions asked in ICAR AIEEA (PG)

Which of the following is an example of a protein domain?

Suspension culture is generally used for

Match List I with List II
List I (Vector):
A. pBR322
B. pUC8
C. pGEM3Z
D. M13mp1

List II (Description):
I. Vector facilitating in vitro transcription
II. Cloning vector based on bacteriophage
III. E. coli plasmid vector to clone DNA
IV. A lac selection plasmid
V. F plasmid based vector for handling longer DNA inserts for genomic library construction

Choose the correct answer from the options given below:

Agar, the gelling agent used for tissue culture media, is obtained from

A transgenic event is differentiated from other events by

Plasmid DNA isolation methods are primarily based on the

Function of ligase is
A) to repair single-stranded breaks that arise in double-stranded DNA
B) to join together two individual fragments of double stranded DNA
Choose the correct answer from the options given below:

Maximum number of types of alleles a recombinant inbred line population derived from crossing two genotypes of rice can generally show at an SSR locus is

Tissue culture for plant transformation should be free from

Which of the following is NOT a millet crop?

Genetic engineering involves the following steps
A. Gene manipulation and vector construction
B. Selection and confirmation of the transformants
C. Gene isolation
D. Genetic transformation
What is the correct sequence in which these steps are performed?

Plasmid vector will essentially have

What types of DNA molecules are copied using rolling circle replication process?

Alkaline phosphatase is used to

Which of the restriction enzyme does produce fragments with blunt end upon restriction digestion?

RNA polymerase tends to bind to which of the following in lac operon system?

Which of the following is NOT an application of plant tissue culture?

Which of the following is most critical temperature, to be decided by primer sequence, in any PCR experiment?

Which of the following library you would prefer to screen to isolate a sequence that encodes a specific protein?

Which of the following is correct extended form of URL?

The biochemical nature of Nuclear Localization Signal is?

Which of the following microorganism can undertake symbiotic relationship with plants?

Which of the following statement is correct for DNA polymerase I of E. coli?
A. Also known as Kornberg polymerase
B. Possesses 5’exonuclease activity
C. Possesses 3’exonuclease activity
D. Plays central role in replication of its genome
Choose the correct answer from the options given below

Which of the following combination is appropriate for hybridoma production?

In order to protect DNA from DNase, which of the following chemical is used during DNA extraction?