List of top Animal Biotechnology Questions on Enzymology asked in ICAR AIEEA (PG)

Match the major class of enzyme with the reaction they catalyze:
A. 1 (Oxidoreductases)
B. 2 (Transferases)
C. 3 (Hydrolases)
D. 4 (Lyases)
Match with the following reactions:
I. Transfer of functional group to water
II. Oxidation-reduction
III. Group transfer
IV. Addition or removal of group to form a double bond
Choose the correct answer from the options given below:

In a reaction mixture of enzyme keeping the enzyme concentration and other conditions constant only substrate concentration is changed. The enzyme activity to corresponding substrate concentrations are given below:
Substrate concentration in mM: 2, 5, 10, 15, 20
Enzyme activity in IU: 15, 25, 50, 56, 58
On the basis of the above observation table, the second-order reaction started after the first-order reaction occurred up to a substrate concentration of:

Which of the following is NOT a correct statement about phospholipase A2?

What are the enzymes in which zinc ion is bound tightly to the enzyme and is not dissociated even after several steps of purification?
A. Carbonic anhydrase
B. Alcoholdehydrogenase
C. Cytochrome oxidase
D. Glucose 6-phosphatase
E. Alkaline phosphatase
F. Lactate dehydrogenase
Choose the most appropriate answer from the option given below

Xanthine oxidase plays an important role in the conversion of purine base to uric acid and it contains

In competitive inhibition
A. Km of the enzyme is increased
B. Vmax of the enzyme is increased
C. Vmax of the enzyme is not changed
D. Inhibitor molecule is structurally similar to the substrate molecule of the enzyme
Choose the correct answer from the option given below:

Citrate is isomerized to isocitrate by the action of enzyme

Given below are two statements:
Statement I: Increase in temperature of a reaction mixture up to an optimum value increases the rate of enzyme reaction as it enhances the rate of collision between the substrate and enzyme active sites
Statement II: Increase in the temperature beyond an optimum value breaks the non-covalent bonds in enzyme first and thereby reaction velocity is decreased
In the light of the above statements, choose the most appropriate answer from the options given below

Given below are two statements:
Statement I: Enzyme can be regulated allosterically if it has at least one ligand-binding site other than the active site
Statement II: Enzyme can be regulated by phosphorylation and dephosphorylation.
In the light of the above statements, choose the most appropriate answer from the options given below

Given below are two statements:
Statement I: Suicidal inhibitor causes covalent modification of an enzyme active site and enzyme permanently becomes inactive
Statement II: Covalent modification of enzyme active site is initiated after binding of substrate to the active site of an enzyme - suicidal inhibitor complex
In the light of the above statements, choose the most appropriate answer from the options given below

If two enzyme E1 and E2 have Km value for a substrate 'A' as 0.1 mM and 0.01 mM respectively, and for another substrate 'B' as 0.5 mM and 0.05 mM respectively, then

Post-mortem autolysis is mainly caused by

Malonate is the competitive inhibitor of

An isoenzyme of hexokinase i.e., glucokinase, is present in

Fluoroacetate inhibit the action of enzyme

Which of the following enzymes is responsible for conversion of testosterone to 17\(\beta\)-estradiol?

Which ONE of the following is an example of an irreversible inhibitor of acetylcholinesterase enzyme?