Question

Identify the correct order of events of enzyme catalyzed reaction.
(A). Formation of ES complex.
(B). Formation of EP complex.
(C). Reaction between E and S.
(D). Release of P and E.
Choose the correct answer from the options given below:

• A. (B), (D), (C), (A)
• B. (D), (A), (C), (B)
• C. (C), (A), (B), (D)
• D. (C), (B), (D), (A)

Hint:

Write out the reaction equation to remember the order:
\[ \text{E} + \text{S} \rightleftharpoons \text{ES} \rightleftharpoons \text{EP} \rightleftharpoons \text{E} + \text{P} \] This matches (C) \(\rightarrow\) (A) \(\rightarrow\) (B) \(\rightarrow\) (D).

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
An enzyme-catalyzed reaction proceeds through a sequential series of binding and chemical transformation steps at the active site.

Step 2: Detailed Explanation:
1. Reaction between E and S (C): The free enzyme (E) and substrate (S) approach each other in solution.
2. Formation of ES complex (A): The substrate binds to the enzyme's active site via non-covalent interactions, forming the Enzyme-Substrate (ES) complex.
3. Formation of EP complex (B): The chemical reaction takes place within the active site, converting the substrate into product and forming the Enzyme-Product (EP) complex.
4. Release of P and E (D): The product (P) dissociates from the active site, releasing the free, unmodified enzyme (E) to participate in another catalytic cycle.
- The correct order of events is (C) \(\rightarrow\) (A) \(\rightarrow\) (B) \(\rightarrow\) (D).

Step 3: Final Answer:
The correct chronological order of events is (C), (A), (B), (D).