List of top Biotechnology Questions on Biotechnology asked in GAT-B

Which of the following is best suited to express large amount of glycosylated protein?
Match List I with List II and choose the correct answer.
The addition of silver nitrate in plant tissue culture media is useful for managing and minimizing detrimental effects of which of the following plant hormones?
The first clinical use of gene therapy was done for treatment of
Which one of the following would, in general, NOT be a preferred choice for transgenic plants?
Given below are two statements:
Statement I: The T-DNA region of a naturally occurring (native) Ti plasmid of Agrobacterium tumefaciens contains genes for biosynthesis of plant hormones.
Statement II: The virulence genes of Agrobacterium tumefaciens are placed on a Helper Plasmid during development of a binary vector system for plant transformation.
In the light of the above statements, choose the correct answer from the options given below
Which is NOT a part of mandatory design considerations of a Rotating Biological Contactor (RBC) system?
Match List I with List II and choose the correct answer.
A monoclonal antibody was heterologously expressed in E. coli and Chinese hamster ovary (CHO) cell line, and the recombinant proteins obtained were purified to homogeneity and were found to be structurally identical. However, the protein expressed in E. coli was not functionally active. The reason could be
A gene whose expression helps to identify transformed cell is known as
Which of the following statement is NOT correct with respect to Cybrids?
Sterilization of fermentation media with steam is performed at
Which of the following bacterium is generally used in plant genetic engineering?
How are healthy plants recovered from diseased plants using plant tissue culture?
Plants which are genetically identical to the original plant from which they are grown are called as
What is Totipotency?
The 'Flavr Savr' tomato was the first genetically engineered crop product commercialized for human consumption. This transgenic tomato is known for:
What is the advantage of using the neo gene to disrupt the function of a gene in knockout mice?
The vector which is most commonly used in crop improvement is:
Given below are few statements related to plant tissue culture:
A. Regeneration frequencies of different types of explants derived from the same plant would always be identical.
B. A higher auxin: cytokinin ratio would induce production of roots under culture conditions.
C. Embryos can be obtained from somatic tissues of plants under in vitro culture conditions.
D. Since plants can photosynthesize, addition of a carbon source in tissue culture media is not advisable.
Given below are two statements:
Statement I: Virulence trait of Agrobacterium tumefaciens is borne on genomic DNA.
Statement II: The technique used to introduce genes in dicot is Ti-plasmid infection.
Why is golden rice pale yellow in color?
Given below are two statements:
Statement I: After immobilization, plant cells are subjected to stress to induce secondary metabolite production.
Statement II: Cells derived from different explants in culture always produce the same set of secondary metabolites.
During protoplast fusion, the product has the nucleus of one of the parents in which the chromosome elimination took place in subsequent cell division and the cytoplasm was contributed by both the parents. Such a product will be called:
Assertion A: Bt toxin does not kill Bacillus thuringiensis.
Reason R: The Bt toxin gets activated due to the alkaline pH of the insect gut.