List of top Biotechnology Questions on Biotechnology

The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 

Which of the following condition(s) lead(s) to reduced affinity of O2 for human hemoglobin?

Which of the following is/are CORRECT for the two molecules shown? 

Match the pair of organisms in Group I with their community interaction pattern in Group II. \[ \begin{array}{c|c} \text{Group I} & \text{Group II} \\ \hline P:\; \text{Cattle egrets – African buffalo} & 3:\; \text{Commensalism} \\ Q:\; \text{Lynx – Fox} & 1:\; \text{Competition} \\ R:\; \text{Acacia tree – Pugnacious ants} & 4:\; \text{Mutualism} \\ S:\; \text{Leopard – Antelope} & 2:\; \text{Predation} \\ \end{array} \]
In a reaction \( A + B \to C \), the initial rate of formation of C at 25°C was measured for different initial concentrations of A and B as given. The overall order of the reaction with respect to both A and B is ................. (answer in integer)
% Requires: \usepackage{graphicx} \begin{table}[h] \centering \begin{tabular}{|c|c|c|c|} \hline Experiment & Initial [A] & Initial [B] & Initial rate of formation of C
& (mol L\textsuperscript{-1}) & (mol L\textsuperscript{-1}) & (mol L\textsuperscript{-1} s\textsuperscript{-1})
\hline 1 & 0.4 & 0.3 & 0.078
2 & 0.8 & 0.3 & 0.312
3 & 0.4 & 0.6 & 0.156
4 & 0.8 & 0.6 & 0.624
\hline \end{tabular} \caption{Experimental data showing initial concentrations and rates of formation.} \label{tab:experimental_data} \end{table} \flushleft \newpage
A buffer solution is composed of 0.1 M acetic acid and 0.15 M sodium acetate. The change in pH of 1 L buffer solution upon addition of 50 mL of 1.0 M NaOH is ............... (rounded off to 2 decimals)
The number of 7 letter words (with or without meaning) starting with the letter B that can be formed using the letters of the word BIOLOGY is .............. (answer in integer)
A candle is placed 18 cm in front of a concave mirror to generate a real, inverted and doubly magnified image. The radius of curvature of the concave mirror is ................ cm. (answer in integer)
The wavelength of a photon emitted during a transition from \( n = 3 \) to \( n = 2 \) state in the H atom is .............. nm. (answer in integer).
Free energy change for transport of an uncharged solute across a membrane against a \(1 \times 10^3\) fold concentration gradient at 25°C is ............... kJ mol\(^{-1}\). (rounded off to 2 decimals) \[ R = 8.315 \, \text{J mol}^{-1} \, \text{K}^{-1} \]
The limit of the function \( \lim_{x \to 2} \frac{2x^2 + 2x - 12}{x^2 - 4} \) is .............. (rounded off to 1 decimal)
Which of the following statement(s) is/are CORRECT about 'stringent response' in bacteria?
Four alkyl halides, MeBr, EtBr, iPrBr, and tBuBr, undergo S\(_N\)2 reactions in the presence of hydroxide ion to yield the corresponding alcohols and the halide ion. The CORRECT order of the alkyl halides based on the rates of reactions is
Which of the following is the CORRECT combination of a synthetic auxin and a synthetic cytokinin?
The 5' cap of eukaryotic mRNAs contains
Which one of the following statements is CORRECT about Agrobacterium tumefaciens-mediated plant transformation?
Which of the following conditions is CORRECT for free expansion of an ideal gas under adiabatic condition? (q = heat, \(\Delta T = \) temperature difference, w = work)
The receptors for gamma amino butyric acid (GABA) in humans are
The IR stretching frequency of the carbonyl (C=O) group of a typical saturated ketone is 1715 cm\(^{-1}\). The IR stretching frequencies for the carbonyl groups present in three different acetophenone derivatives are given. Match the molecules in Group I with their corresponding frequencies in Group II. \[ \begin{array}{c|c} \text{Group I} & \text{Group II (C=O stretching frequency cm}^{-1}\text{)}
\hline P:\; p\text{-amino acetophenone} & 1:\; 1677
Q:\; p\text{-nitro acetophenone} & 2:\; 1700
R:\; p\text{-methoxy acetophenone} & 3:\; 1684
\end{array} \]
Two charges \(10 \times 10^{-8}\,\text{C}\) and \(-6 \times 10^{-8}\,\text{C}\) are located 16 cm apart. The point(s) on the line joining the two charges, where the net electric potential is zero, will be [Take the potential at infinity to be zero]
Bacterial cloning vectors and bacterial expression vectors are differentiated by the presence of
Which of the following activities is NOT possessed by the human immunodeficiency virus-1 reverse transcriptase?
What is the ploidy level of a sporophyte in angiosperms?
The standard free energy change (\(\Delta G^0\)) of the binding reaction between different sweet molecules and a common sweet taste receptor are given. Which amongst these molecules is the sweetest at the same molar concentration?
The correct order of electronegativity of the given elements is