List of top Botany Questions on Biotechnology

Which enzyme is used to join two DNA fragments in genetic engineering?

Choose the correct statements among the following
I. Streptokinase is produced by Streptococcus.
II. Saccharomyces cerevisiae is used for commercial production of Ethanol.
III. Cyclosporin-A — blood cholesterol lowering agent produced by Trichoderma polysporum.
IV. Statin produced by yeast.

Match the following

List - I	List - II
A. Ori	IV. Controlling the copy number of linked DNA
B. Selectable marker	III. Permitting the growth of transformants
C. Cloning site	II. Single recognition site to link alien DNA
D. Cloning vector	I. Low molecular weight

Choose the correct statements among the following
I. Pure DNA can be isolated by using ribonuclease and proteases.
II. Ethidium bromide staining facilitates the visualisation of DNA fragment in Agarose gel.
III. Sticky and ligation techniques help to cut the gene of interest from the source DNA.

Choose the correct statement among the following
I. Validity of GM research and safety of introducing GMO has to be given by GEAC.
II. Molecular farming means utilization of plants as biofactories for obtaining commercially useful products.
III. Basmati Rice variety is a transgenic plant with improved nutritional value.
IV. “Flavr savr” is suitable for food storage technology.

Select the correct statements among the following
I. The use of biofertilizers reduce the use of chemical fertilizers.
II. Mycorrhizae enhances phosphorous content in the soil.
III. An important biofertilizer in legume crops is Rhizobium.
IV. Glomus forms mycorrhizal association with plants.

Match the following:

Study the following lists: