List of top Botany Questions on Applications of Biotechnology

Choose the incorrect statements:

I. Human insulin is made in fungus
II. Insulin DNA sequences are introduced into the genophore of E. coli
III. Recombinant E. coli produces insulin chains

Which of the following is not a biosafety concern for GM crops?

Choose the correct pair for artificially restructured E. coli cloning vector
I. pBR322 - Boliver and Rodrigues - Natural Vector
II. Selectable marker - Antibiotics - amp", terR
III. Restriction sites - Hind III, Pst I - Link alien DNA
IV. roP - Proteins - supports high copy number

Identify the correct combination.
A. Agrobacterium tumefaciens – vector for transgenic plant.
B. Escherichia coli – selectable marker.
C.Thermus aquaticus – Taq polymerase.
D.Bacillus thuringiensis – BT. Toxin.

Assertion (A): Bio piracy is use of bio resources by multinational companies with proper authorization from the other countries and people.
Reason (R): Industrialized nations are poor at biodiversity but rich financially.

Assertion (A): Bioterrorism involves the actual use or threat to use biological agents to spread fear or inflict death and death of large populations.
Reason (R): It paves way for the development of new vaccines and therapies.

The transgenic Flavr Savr tomato carries cloned genes for
A. delay in ripening process
B. longer shelf life
C. added flavors
D. vitamin A

Among the following, the wrong statement related to Bt cotton

The introduction of nematode-specific genes into host using Agrobacterium vectors causes the following action:
I. Formation of dsRNA.
II. Production of sense and antisense RNAs in the host cells.
III. RNA intermediate not formed.
IV. Silencing of specific mRNA of the nematode.