Question:

Choose the correct pair for artificially restructured E. coli cloning vector
I. pBR322 - Boliver and Rodrigues - Natural Vector
II. Selectable marker - Antibiotics - amp", terR
III. Restriction sites - Hind III, Pst I - Link alien DNA
IV. roP - Proteins - supports high copy number

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E. coli cloning vectors often include selectable markers and restriction sites for inserting foreign DNA.
Updated On: Mar 19, 2026
  • I & IV
  • II & III
  • III & IV
  • II & IV
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The Correct Option is B

Solution and Explanation

The correct pair is: - II: Selectable marker – Antibiotics (amp\(^R\), tet\(^R\))
- III: Restriction sites – Hind III, Pst I – Link alien DNA
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