List of top Biology Questions on Recombinant DNA technology and applications

Arrange the following steps in recombinant DNA technology on the basis of their sequence of occurrence:
(A) Isolation of desired DNA fragments
(B) Culturing the host cells
(C) Extraction of desired product
(D) Ligation of DNA fragment into vector
(E) Transferring of recombinant DNA to host
Choose the correct answer from the options given below:

Transposons are:

Match the type of DNA repair mechanism in Group I with the enzyme(s) involved in Group II

Group I	Group II
P) Mismatch repair	1) DNA glycosylase
Q) Base excision repair	2) UvrA, UvrB, UvrC and UvrD
R) Nucleotide excision repair	3) RecA
S) Double strand break repair	4) MutL, MutS and MutH

In the ‘Southern blot’ technique, which of the following reagents is used to detect the presence of a desired DNA fragment?

Which technique is used to insert recombinant DNA into the nucleus of an animal cell ?

From the following tools / techniques of genetic engineering, identify those which are required for cloning a bacterial gene in animal cells and choose the correct option :

I.	Endonuclease	II.	Ligase
III.	tumefaciens	IV.	Microinjection
V.	Gene gun	VI.	Lysozyme
VII.	Cellulase	VIII.	Electrophoresis

Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement. (1) (2)(3) (4)

An analysis of chromosomal DNA using the southern hybridisation technique does not use:

Commonly used vectors for human genome sequencing are:

Which vector can clone only a small fragment of DNA?

In vitro clonal propagation in plants is characterized by:

If a recombinant DNA bearing gene for resistance to Ampicillin transferred into E.coli cells, host cells become transformed into Ampicillin resistant cells. What happens when these E.coli are grown on medium containing Ampicillin?