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Which one of the following statements is not true regarding gel electrophoresis technique?
Which one of the following statements is not true regarding gel electrophoresis technique?
Updated On: May 4, 2024
- The process of extraction of separated DNA strands from gel is called elution
- The separated DNA fragments are stained by using ethidium bromide
- The presence of chromogenic substrate gives blue coloured DNA bands on the gel
- Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light
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The Correct Option is C
Solution and Explanation
Gel electrophoresis does not employ chromogenic substrates; instead, the DNA is made visible by intercalating it with EtBr, which can be viewed under UV light. Without UV illumination, DNA bands are not discernible in the gel.
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.
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