Question:

In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

Updated On: Aug 5, 2024
  • Zinc

  • Tungsten or gold

  • Silver

  • Copper

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The Correct Option is B

Solution and Explanation

Tungsten or gold microparticles are commonly used in the gene gun method for gene transfer. The microparticles are coated with the foreign DNA of interest and are then propelled into the target cells using a biolistic device (gene gun). The high velocity of the microparticles enables them to penetrate the cell membranes and deliver the foreign DNA into the host cells, allowing for genetic transformation or transfection.

Therefore, The correct option is (B): Tungsten or gold

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Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.