Question

Which of the following methods of separation are applicable to Liquid Chromatography (LC)? A. Liquid--liquid B. Size exclusion C. Gas--solid D. Ion exchange E. Affinity

• A. A and B only
• B. C, D and E only
• C. A, B, D and E only
• D. A, B and C only

Hint:

LC methods: liquid--liquid, size exclusion, ion exchange, affinity, adsorption. Gas--solid belongs to GC, not LC.

Answer 1

The Correct Option is C

Step 1: Concept
Types of separation mechanisms applicable to Liquid Chromatography (LC).

Step 2: Meaning
LC uses a liquid mobile phase; the stationary phase and interaction type define the separation method.

Step 3: Analysis
Liquid--liquid (A): Partition chromatography using two immiscible liquids --- applicable to LC.
Size exclusion (B): Gel filtration/permeation; separates by molecular size --- applicable to LC.
Gas--solid (C): Requires a gaseous mobile phase --- applicable to Gas Chromatography, not LC.
Ion exchange (D): Uses ion-exchange resins with liquid mobile phase --- applicable to LC.
Affinity (E): Uses biological affinity interactions with liquid mobile phase --- applicable to LC.

Step 4: Conclusion
A, B, D, and E are applicable to LC; C (gas--solid) is not. Final Answer: (C)