Question:
Which among the following is a primary binding/serological test
Which among the following is a primary binding/serological test
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Serological Test Tiers:
Primary Binding $\rightarrow$ ELISA, RIA, IFA (Direct tagged measurement).
Secondary $\rightarrow$ Agglutination, Precipitation, Complement Fixation.
Tertiary $\rightarrow$ In vivo protection tests.
Primary Binding $\rightarrow$ ELISA, RIA, IFA (Direct tagged measurement).
Secondary $\rightarrow$ Agglutination, Precipitation, Complement Fixation.
Tertiary $\rightarrow$ In vivo protection tests.
Updated On: Sep 7, 2026
- ELISA
- Bacterial Agglutination
- Complement Fixation
- Gel Precipitation
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Serological assays are categorized into primary binding tests, secondary (lattice-formation) tests, and tertiary in vivo biological assays.
Step 2: Detailed Explanation:
Classification of serological assays:
1. Primary Binding Assays: Directly measure the initial physical binding event between antibody paratope and antigen epitope using tagged tracer molecules (enzymes, radioisotopes, fluorophores). Examples include ELISA (Enzyme-Linked Immunosorbent Assay), Radioimmunoassay (RIA), and Immunofluorescence Assays (IFA).
2. Secondary Binding Assays: Depend on secondary macroscopic lattice formation or biological consequences following primary binding. Examples include Agglutination, Precipitation (Gel diffusion), and Complement Fixation Tests (CFT).
3. Tertiary Assays: In vivo toxin neutralization or protection tests.
Step 3: Final Answer:
Therefore, ELISA is a primary binding/serological test, corresponding to option (A).
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