Question:
Klenow fragment is the modified enzyme of which DNA polymerase:
Klenow fragment is the modified enzyme of which DNA polymerase:
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Klenow = DNA Pol I minus its âFront-endâ ($5'\to3'$) exonuclease.
Updated On: May 28, 2026
- DNA polymerase I
- DNA polymerase II
- DNA polymerase III
- DNA polymerase IV
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The Correct Option is A
Solution and Explanation
Step 1: Concept
DNA Polymerase I in E. coli has three activities: $5'\to3'$ polymerase, $3'\to5'$ exonuclease, and $5'\to3'$ exonuclease.
Step 2: Meaning
The Klenow fragment is produced when DNA Polymerase I is treated with the protease subtilisin.
Step 3: Analysis
This cleavage removes the $5'\to3'$ exonuclease domain, leaving a large fragment retaining polymerase and proofreading functions.
Step 4: Conclusion
The Klenow fragment is specifically a derivative of DNA Polymerase I.
Final Answer: (1)
DNA Polymerase I in E. coli has three activities: $5'\to3'$ polymerase, $3'\to5'$ exonuclease, and $5'\to3'$ exonuclease.
Step 2: Meaning
The Klenow fragment is produced when DNA Polymerase I is treated with the protease subtilisin.
Step 3: Analysis
This cleavage removes the $5'\to3'$ exonuclease domain, leaving a large fragment retaining polymerase and proofreading functions.
Step 4: Conclusion
The Klenow fragment is specifically a derivative of DNA Polymerase I.
Final Answer: (1)
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