Question

If the Vmax of an enzyme is 10 IU and its Km is 20 mM then Lineweaver Burk plot of this enzyme follows a slope of:

• A. 2.0
• B. 1.0
• C. 0.5
• D. 0.25

Hint:

Lineweaver-Burk plot: 1/V vs 1/[S].
Slope = Km/Vmax.
Intercept = 1/Vmax.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
Lineweaver-Burk plot is a double reciprocal plot.
It is used to determine Km and Vmax.

Step 2: Key Formula or Approach:
Lineweaver-Burk equation: 1/V = (Km/Vmax) \(\times\) 1/[S] + 1/Vmax.
Slope = Km/Vmax.

Step 3: Detailed Explanation:
Given: Vmax = 10 IU, Km = 20 mM.
Slope = Km Vmax = 20 10 = 2.0.
Thus, the slope of the Lineweaver-Burk plot is 2.0.

Step 4: Final Answer:
The slope of the Lineweaver-Burk plot is 2.0.
Hence, the correct option is (A).