Step 1: Write the molecular formula of glycine.
Glycine is the simplest amino acid and its molecular formula is
\[
C_2H_5NO_2
\]
Step 2: Calculate the molecular mass of one glycine molecule.
Using the given atomic masses:
\[
C=12,\quad H=1,\quad N=14,\quad O=16
\]
Mass of glycine:
\[
=2(12)+5(1)+1(14)+2(16)
\]
\[
=24+5+14+32
\]
\[
=75
\]
Thus, molecular mass of glycine is
\[
75\ \text{Da}
\]
Step 3: Find the mass of three glycine molecules.
A tripeptide contains three glycine units.
\[
3\times 75=225
\]
So, total mass before peptide bond formation is
\[
225\ \text{Da}
\]
Step 4: Understand peptide bond formation.
When amino acids join to form peptide bonds, one water molecule
\[
(H_2O)
\]
is removed for each peptide bond formed.
Step 5: Determine number of peptide bonds.
A tripeptide formed from three amino acids contains
\[
2
\]
peptide bonds.
Hence, two water molecules are lost.
Step 6: Calculate mass loss due to water removal.
Mass of one water molecule:
\[
H_2O=2(1)+16=18
\]
Mass of two water molecules:
\[
2\times18=36
\]
Now subtract from total mass:
\[
225-36=189
\]
Step 7: Final conclusion.
Therefore, the molecular mass of the tripeptide is
\[
\boxed{189}
\]