List of top Animal Biotechnology Questions on Biochemical Techniques asked in ICAR AIEEA (PG)

In SDS-PAGE, the proteins are separated mainly based on

Which of the following reagents specifically react with amino-terminal amino acid present in a peptide?

In ion-exchange chromatography, the charge on the anion exchangers is

Identify the strong anion exchangers among the following exchange types commonly used in ion-exchange chromatography techniques.

Centrifugation technique which uses CsCl for the separation of DNA is known as

Match List-I with List-II:

Choose the correct answer from the options given below:

Match List-I with List-II:

Choose the correct answer from the options given below:

Match List-I with List-II:

Choose the correct answer from the options given below:

Match List-I with List-II:

Choose the correct answer from the options given below:

Match List-I with List-II
List-I (Name of the Fluorescent Dye) vs List-II (Colour of the Fluorescent Emission)
(A) Amino methyl coumarin - (I) Red
(B) Rhodamine - (II) Green
(C) Flourescien - (III) Blue green
(D) Allophycocyanin - (IV) Blue
Choose the correct answer from the options given below:

Which of the following methods are used to label the double stranded DNA?
(A) 5' - end labelling with polynucleotide kinase
(B) 3' - end labelling with polynucleotide kinase
(C) 5' - end labelling with terminal transferase
(D) 3' - end labelling with terminal transferase
Choose the correct answer from the options given below:

Which RNA molecule with the following feature used for cDNA synthesis?

Which of the following parameters affect the annealing of primer to the template in PCR?
(A) Nucleotide sequence of the primer.
(B) Temperature of annealing.
(C) Specificity of Taq DNA Polymerase.
(D) GC content of the template.
(E) Concentration of $\text{Mg}^{2+}$ ions.
Choose the correct answer from the options given below:

Which of the following process helps to ascertain the specificity of amplification in the case of realtime PCR technique?

Name a liver specific enzyme primarily for the large domestic species and is also a zinc metalloenzyme.

Which technique is used to visualize the distribution of protein in the membrane ?

To which one of the following groups would it be acceptable to give a live attenuated viral vaccine?

If a splice site were mutated so that splicing did not take place, what would be the effect on the mRNA?

First NIH guidelines for the conduct of recombinant DNA research were issued in which year?

The 4-amino or 4-keto group of pyrimidine bases is located in the

A DNA strand with sequence 5' CGATTC 3' would be complementary to the sequence

Which of the following is responsible for increase in anion gap during diabetic ketosis?

Which cell organelle contains the hydrolase enzyme cathepsin ?

A stem cell transplant using a patient's own stem cells is described as:

Ribozymes were discovered by: