List of top Biochemistry Questions asked in GATE Life Sciences

Which of the following fatty acids is/are known to increase the low density lipoprotein (LDL)-cholesterol?

Match the anti-nutritional factor in Column I with their corresponding activity given in Column II.

Column I		Column II
(P)	Lectin	1	Total amount of produced by autotrophs energy
(Q)	Stachyose	2	Chelates with divalent cations and reduces their bio-availability
(R)	Phytate	3	Inhibits trypsin and chymotrypsin
(S)	Knuitz type inhibitor	4	Hemagglutination

Xeropthalmia is caused due to the deficiency of

Which one of the following statements is NOT TRUE of glycosaminoglycans?

When the blood glucose level of a healthy person is 100 mg/dL, which one of the following options is most likely to represent the level of glucose in the urine of that person?

A fluorescence microscope with an objective lens of numerical aperture (NA) 1.5 is used with light of wavelength (λ) 600 nanometers. The lateral resolution limit of this microscope rounded off to the nearest integer, is______nanometers.

Which one of the following statements about gene expression is INCORRECT?

Which of the following is/are active transport mechanism(s) in prokaryotes where the substance is chemically altered during transport across the membrane?

At root nodules, which of the following C4 organic acid(s) is/are transported across the symbiosome membrane and into bacteroids?

Which one of the following double-stranded sequences will NOT be recognized by a Type IIP restriction endonuclease?

Match the inhibitors in Group 1 with their respective targets in Group 2.

Group 1	Group 2
P) Oligomycin	(i) Cytochrome bc1 complex
(Q) Antimycin A	(ii) Photosystem II
(R) 3-(3,4-dichlorophenyl)-1, 1- dimethylurea (DCMU)	(iii)K\(^+\) ionophore
(S) Valinomycin	(iv) Fo ATP synthase

In Calvin-Benson cycle, to produce 1 molecule of glyceraldehyde 3-phosphate by fixing 3 molecules of carbon dioxide, 9 molecules of ATP and____ molecules (in integer) of NADPH are typically utilized.

A 5250 base-pair long plasmid with 10 negative supercoils would have a linking number of______,considering 10.5 base pairs per turn for B DNA. (Answer in integer)

The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 2960.6 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 2960.6 peak is____ Da. (Round off to two decimal places)

Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?

Which one or more of the following statements correctly describe(s) the addition of N-nucleotides during the rearrangement of the immunoglobulin heavy chain- encoding gene?

Which one among the following structures is the most stable conformer of (Z)-pent-2-ene?

An enzyme-catalyzed conversion of a substrate at 298 K proceeds by a Michaelis-Menten mechanism. The Lineweaver-Burk plot for the analysis of the experimental data has an intercept along the y-axis of 0.357 mmol\(^{-1}\) dm\(^{3}\) s and a slope of 2.10 s. The correct Michaelis constant for the reaction is ______ (rounded off to 2 decimal places).

Match the inhibitors in Group 1 with their respective targets in Group 2.

Group 1	Group 2
P) Oligomycin	(i) Cytochrome bc1 complex
(Q) Antimycin A	(ii) Photosystem II
(R) 3-(3,4-dichlorophenyl)-1, 1- dimethylurea (DCMU)	(iii)K\(^+\) ionophore
(S) Valinomycin	(iv) Fo ATP synthase

In Calvin-Benson cycle, to produce 1 molecule of glyceraldehyde 3-phosphate by fixing 3 molecules of carbon dioxide, 9 molecules of ATP and____ molecules (in integer) of NADPH are typically utilized.

The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 2960.6 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 2960.6 peak is____ Da. (Round off to two decimal places)

Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?

A 5250 base-pair long plasmid with 10 negative supercoils would have a linking number of______,considering 10.5 base pairs per turn for B DNA. (Answer in integer)

Which one or more of the following statements correctly describe(s) the addition of N-nucleotides during the rearrangement of the immunoglobulin heavy chain- encoding gene?

Which one among the following structures is the most stable conformer of (Z)-pent-2-ene?