List of top Biotechnology Questions on Techniques in Biotechnology

What is the full form of RFLP? State its basic principle and mention any two important applications.

Describe the steps involved in a Polymerase Chain Reaction (PCR) technique.

If a researcher starts with three double stranded DNA molecules, how many such molecules will be produced after 10 PCR cycles? Explain briefly.

Briefly describe Nick Translation technique.

The technique of Nick Translation was developed by:

Explain the steps involved in PCR amplification method.

Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.

A protein ion with a molecular weight of 10,000 Daltons carried a charge of 5+ and was subjected to mass spectrometric analysis. Calculate its mass to charge ratio.