List of top Biotechnology Questions on Recombinant DNA technology and applications

Describe the basic steps of Recombinant DNA Technology.
Explain the method of Blue-White selection used for screening of recombinant cells containing desired plasmid with gene of interest.
Outline the important steps for isolation of recombinant insulin (Humulin) from Escherichia coli.
The DNA ligase enzyme that is frequently used to ligate different DNA fragments in order to generate rDNA molecules is isolated from:
Which is correct regarding ex vivo gene therapy?
Which is an endonuclease in CRISPR Cas9 Technique?
Which technique is not used for site directed mutagenesis?
Which vector is not suitable for Genomic DNA library construction?
The correct sequence of enzymes used for cDNA library preparation is

For expression of eukaryotic gene in a prokaryotic system, which sequence has to be inserted from its first codon?

Which among the following is incorrect with reference to Yeast Artificial Chromosomes?
pBluescript II KS is a
Identify the vector which is not a phage vector among the following?
Which type of restriction enzymes are used in genetic engineering work
Recombinant proteins are often produced on a large scale using
Which technique is used to separate and analyze DNA fragments that are of different lengths?
Which of the following is NOT a thermostable polymerase?
Which promoter is used for yeast cell expression?
Which of these would not be used to introduce DNA in animal cells?
Which of the following restriction enzyme produce blunt ends?
Isoschizomers recognize ..................
All these statements are true regarding RFLP and RAPD except
Premature or incomplete protein synthesis happens due to:
The variation in the number of tandem repeats between two or more individuals is called:
The concept of DNA computing was kick-started by the famous scientist: