List of top Biotechnology Questions on Molecular Biology

Write the difference between dNTPs and ddNTPs. What is the function of ddNTPs in Sanger’s method of DNA sequencing?
Describe the procedure involved in Restriction Fragment Length Polymorphism (RFLP) technique. Why is this technique used in forensic sciences?
Which enzyme is used in Sanger’s method of DNA sequencing?
What is the advantage of using ddNTPs conjugated with fluorescent molecules in automated method of DNA sequencing?
Why are primers required in Sanger’s method of DNA sequencing?
Why is chronic myelogenous leukemia caused? Name the technique used to know the status of this disease.
At the end of one cycle of PCR, two DNA molecules will become ________ .
Site-directed mutagenesis is commonly used to:
Which of the following techniques uses temperature cycling for DNA amplification?
Which of the following vectors can accommodate the largest foreign DNA insert?
The plasmid pBR322 contains two antibiotic resistance genes:
Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?
Which one of the following tools of recombinant DNA technology is INCORRECTLY paired with its applications?
The central dogma of molecular biology describes:
In DNA replication, the leading strand is synthesized:
Which of the following statements about prions is true?
Which enzyme allows retroviruses to transcribe RNA into DNA?
Which blotting technique is used for detection of proteins?
Which one makes correct components of a PCR reaction?
Which is incorrect with reference to transposon?

Match the following

XRibosome binding site1Enhance transcription
YPromoter2Shine Dalgarno Sequence
ZEnhancer3Molecular Switches to turn on transcription
Nucleosome a repeating structural unit within eukaryotic chromatin is made up of
Which sequencing technique relies on the synthesis of complementary strands in the presence of chain-terminating dideoxynucleotides?
Which among the following is true for RFLP?
Hardy-Weinberg Law / equilibrium model in population genetics means