Which of these statements is/are correct about competitive ELISA?
(A). It can be used for accurate measurement of the circulating level of the hormone.
(B). There is competition between the natural antigen (hormone) to be tested for and an enzyme conjugated form of the antigen which is the detection reagent.
(C). The more natural antigen present the more it will displace (compete out) the conjugated form leading to a reduction in enzyme bound to the plate.
(D). The more natural antigen bound to the antigen, the higher the signal will be generated.
Choose the correct answer from the options given below: