List of top Animal Biotechnology Questions on Molecular Biology

Which of the following describes the mode of action of Tetracycline as an antibiotic?

A 2 kb DNA fragment was cloned into BamHI and EcoRI sites located on a 8 kb cloning vector. The BamHI and EcoRI sites are adjacent to each other on the vector backbone. The vector contains an XhoI site located 300 bp upstream of the BamHI site. An internal XhoI site is present in the gene sequence. The resultant recombinant plasmid is digested with EcoRI and XhoI and analyzed through 1% agarose gel electrophoresis. Assuming complete digestion with EcoRI and XhoI, the DNA fragments (in base pairs) visible on the agarose gel will correspond to:

Caenorhabditis elegans has a genome size of 103Mb, how many number of genes (approx.) are there in its genome?

Under certain circumstances, an elongating RNA polymerase can become arrested and cease transcribing. To deal with this situation, the cell has machinery that removes the arrested polymerase and at the same time recruits repair enzymes. Name the enzyme(s) which is part of the machinery.

The serum in mammalian cell culture medium stimulates cell growth and attachment. However, it must be filter sterilized prior to use to

Which of the following statement is correct about DNA vaccines?

Recently, it was reported that adult, differentiated cells can be made to behave as pluripotent stem cells by the introduction of a few genes, one of which was Oct What was the rationale for this experiment?

Identify the correct order of events when epinephrine binds to \(\beta\)-receptor of the biological membrane.
A. Binding of GTP and release of GDP from \(\alpha\)-subunit of G-Protein.
B. Interaction of hormone bound \(\beta\)-receptor with \(\beta\gamma\) - subunit of G-Protein.
C. Binding of GTP bound \(\alpha\)-subunit of G-protein with Adenyl cyclase
D. Activation of dephosphorylation
E. Activation of phosphorylation
F. Activation of formation of cAMP
Choose the correct answer from the options given below:

Which of the following hormones are amino acid derivatives?
A. Epinephrine
B. Serotonin
C. GABA
D. Thyroxine
E. Vasopressin
Choose the correct answer from the options given below:

Match the following toxic chemicals with the suitable conjugants for the process of detoxification.
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Choose the correct answer from the options given below:

Hershey and Chase designed an experiment using radioactive isotopes of sulphur and phosphorus to keep separate track of the viral proteins and nucleic acids during the infection process. Which bacteriophage and bacterium were used for this experiment?

Caesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, \(\rho\), of a double stranded Cs+DNA is given by the equation \(\rho = 66 + 0.098 X_{G+C}\), where \(X_{G+C}\) denotes

Which of the following enzyme is considered to be liver specific in the case of dogs and cats, but not clinically significant in horses and ruminants?

In Protein Kinase A, the letter ‘A’ refers to

Among the following, identify the hormones produced by the anterior pituitary which is glycoprotein in nature
A. Somatotropin
B. Adrenocorticotropic Hormone
C. Thyroid Stimulating Hormone
D. Luteinizing Hormone
Choose the correct answer from the options given below:

Lipoxygenase pathway leads to formation of

Name the bacterial and yeast polysaccharide that is found in dental plaque on the surface of teeth.

Which disease is caused due to absence of the enzyme hexosaminidase A and results in accumulation of ganglioside \(G_{M2}\) in the brain.

Why the food product gelatine, derived from collagen has little nutritional value as a protein?

In lac operon, which enzyme is NOT directly involved in lactose metabolism?

Which of the following does NOT require a primer in order to function?

Recombinant vaccines are also referred to as:

Which glycoprotein promotes attachment of cells to the substrate?

Which of the following PCR is used to amplify an unknown sequence of DNA if only a single sequence is known?

Presence of adequate amount of glucose in medium prevents the full expression of genes specifying proteins involved in fermentation of other metabolites in E. coli. This phenomenon is called: