Question:
Which one of the following is a nucleic acid based method for the detection of pathogens /their toxins?
Which one of the following is a nucleic acid based method for the detection of pathogens /their toxins?
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DNA/RNA hybridization, PCR, and RT-qPCR are molecular nucleic acid assays, whereas ELISA and RPLA are antigen-antibody serological assays.
Updated On: Sep 15, 2026
- Hybridization
- Bioluminescence
- Electrochemical immunosensors
- Reverse Passive Latex Agglutination
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Pathogen detection methods are broadly categorized into culture-based, immunological, and nucleic acid-based molecular methods.
Step 2: Detailed Explanation:
Nucleic acid hybridization relies on the specific hydrogen bonding and pairing between a labeled single-stranded DNA/RNA probe and complementary target microbial gene sequences.
In contrast, bioluminescence measures ATP via luciferase, electrochemical immunosensors use antibodies, and Reverse Passive Latex Agglutination (RPLA) is an antibody-coated latex agglutination assay.
Step 3: Final Answer:
Hence, hybridization is the nucleic acid-based method for pathogen detection.
Pathogen detection methods are broadly categorized into culture-based, immunological, and nucleic acid-based molecular methods.
Step 2: Detailed Explanation:
Nucleic acid hybridization relies on the specific hydrogen bonding and pairing between a labeled single-stranded DNA/RNA probe and complementary target microbial gene sequences.
In contrast, bioluminescence measures ATP via luciferase, electrochemical immunosensors use antibodies, and Reverse Passive Latex Agglutination (RPLA) is an antibody-coated latex agglutination assay.
Step 3: Final Answer:
Hence, hybridization is the nucleic acid-based method for pathogen detection.
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