Question:
Which of the following is a fluorescent stain?
A. Indian Ink
B. Rose Bengal
C. Chromomycin A3
D. Eosin
Choose the correct answer from the options given below:
Which of the following is a fluorescent stain?
A. Indian Ink
B. Rose Bengal
C. Chromomycin A3
D. Eosin
Choose the correct answer from the options given below:
A. Indian Ink
B. Rose Bengal
C. Chromomycin A3
D. Eosin
Choose the correct answer from the options given below:
Show Hint
Fluorescent DNA Stains: Chromomycin A3 (GC-specific), DAPI (AT-specific), Hoechst 33342, Propidium Iodide, Ethidium Bromide, Acridine Orange.
Updated On: Sep 7, 2026
- A only
- A and B only
- C only
- All A, B, C and D
Show Solution
Verified By Collegedunia
The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept:
Fluorochromes and fluorescent DNA base-specific dyes in cytogenetics and sperm chromatin structure assays.
Step 2: Detailed Explanation:
Evaluating biological dyes and fluorochromes:
1. Chromomycin A3 (C only): A guanine-cytosine (GC) base-specific fluorochrome (fluorescent dye) that binds to DNA minor grooves and fluoresces bright yellow under ultraviolet excitation. Used in flow cytometry and sperm chromatin structure assays to evaluate sperm DNA protamination and condensation.
2. India Ink: Colloidal carbon suspension used for negative background staining of capsules.
3. Rose Bengal: Non-fluorescent biological stain agglutination dye.
4. Eosin: Standard pink acidic histological counterstain.
Thus, Chromomycin A3 (C only) is the fluorescent stain.
Step 3: Final Answer:
Thus, the fluorescent stain is C only (Chromomycin A3), matching option (C).
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