Step 1: Understanding the Concept:
Superovulation is the pharmacological induction of multiple follicular ovulations in a single estrous cycle.
It is the cornerstone of embryo transfer technology in cattle, aiming to maximize the yield of viable embryos from donor cows.
Step 2: Detailed Explanation:
To induce superovulation, exogenous hormones with strong follicle-stimulating activity are administered to rescue antral follicles from atresia:
- A. Follicle Stimulating Hormone (FSH): This is a pituitary gonadotropin that binds to FSH receptors on granulosa cells, promoting follicular development and recruitment. It is the most common hormone used for superovulation.
- B. Pregnant Mare Serum Gonadotropin (PMSG eCG): Produced by endometrial cups in pregnant mares, it possesses strong FSH-like activity in other species due to its high carbohydrate content and long half-life. It is used as an alternative agent to induce superovulation.
- C. GnRH (Gonadotropin Releasing Hormone) is used to induce LH surge and ovulation, not to recruit multiple follicles.
- D. Progesterone is used for estrus synchronization, not follicle stimulation.
- E. Estrogen is used to manipulate follicular waves or synchronize estrus, but cannot directly induce superovulation.
Thus, only FSH and PMSG are utilized as superovulating agents.
Step 3: Final Answer:
The hormones used for superovulation are FSH and PMSG (A and B only).