Question:
Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below:
Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below:
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below:
Updated On: Apr 30, 2026
- B, C, E only
- A, D, E only
- A, C, D only
- B, C, D only
Show Solution
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The Correct Option is D
Solution and Explanation
To solve the question regarding EcoR1 and determine which options are correct, let us explore the characteristics and functions of EcoR1:
- EcoR1 and DNA Cutting: EcoR1 is an endonuclease, not an exonuclease, meaning it cuts within the DNA strand rather than removing nucleotides from the ends. It specifically cuts the DNA at a recognition site, which is often palindromic.
- Recognition Sequence: EcoR1 recognizes the sequence \text{GAATTC} . This is a palindromic sequence, meaning it reads the same backward as forward on complementary strands. Thus, option C is correct.
- Sticky Ends vs. Blunt Ends: EcoR1 creates sticky ends, not blunt ends. These sticky ends contain overhangs that make it easier for DNA fragments to align and hybridize, which is crucial for cloning. This eliminates option A and confirms option B.
- Cutting Specificity: EcoR1 cuts between G and A within its recognition site, \text{GAATTC} , making option D correct. The cut leads to sticky ends with unpaired nucleotides.
- Exonuclease Activity: As mentioned, EcoR1 is an endonuclease, not an exonuclease. Hence, option E is incorrect.
Based on the above analysis, the correct answer is B, C, D only, as these options accurately describe the properties and actions of EcoR1.
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