Question:

Which of the following activities is usually NOT performed during RNA processing in a eukaryotic cell?

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hnRNA processing includes: (1) 5' 7-methylguanosine capping, (2) 3' Poly-A tailing, (3) Intron splicing (exons are retained).
  • Addition of 5'cap
  • Elimination of exons
  • Addition of poly-A tail
  • Elimination of introns
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the Concept:
Eukaryotic primary transcripts (hnRNA) undergo co-transcriptional and post-transcriptional processing to yield mature functional mRNA.

Step 2: Detailed Explanation:

Standard eukaryotic mRNA processing steps include:
1. 5' Capping: Addition of 7-methylguanosine ($m^7G$) cap to the 5' end.
2. 3' Polyadenylation: Cleavage and addition of a poly-A tail ($pprox 200\text{--}250$ adenylate residues) to the 3' end.
3. RNA Splicing: Precise excision and elimination of non-coding intervening sequences (introns) and covalent ligation of coding sequences (exons).
Exons are preserved and spliced together; they are not eliminated during standard processing.

Step 3: Final Answer:

Hence, elimination of exons is NOT performed.
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