Question:
Which enzyme is used to join the ends of two strands of DNA?
Which enzyme is used to join the ends of two strands of DNA?
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Remember: Restriction endonucleases are molecular scissors (they cut DNA), whereas DNA ligases are molecular glue (they join DNA).
Updated On: Jul 8, 2026
- Ligase
- Synthetase
- Polymerase
- Restriction endonuclease
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
In molecular biology and biotechnology, specific enzymes are used to manipulate DNA strands by cutting, synthesizing, or joining them.
Step 2: Detailed Explanation:
- DNA Ligase: This is the molecular "glue" of the cell. It catalyzes the formation of a covalent phosphodiester bond between the 3'-hydroxyl group of one DNA strand and the 5'-phosphate group of another. It is used to join Okazaki fragments during DNA replication and to ligate gene inserts into vectors during recombinant DNA procedures.
- Synthetase: General class of enzymes that catalyze the synthesis of molecules, often using energy from ATP.
- DNA Polymerase: Synthesizes new DNA strands by adding free nucleotides complementary to a template strand.
- Restriction Endonuclease: Functions as molecular "scissors" that cut DNA at specific nucleotide sequences.
Step 3: Final Answer
DNA ligase is the enzyme used to join the ends of two DNA strands.
In molecular biology and biotechnology, specific enzymes are used to manipulate DNA strands by cutting, synthesizing, or joining them.
Step 2: Detailed Explanation:
- DNA Ligase: This is the molecular "glue" of the cell. It catalyzes the formation of a covalent phosphodiester bond between the 3'-hydroxyl group of one DNA strand and the 5'-phosphate group of another. It is used to join Okazaki fragments during DNA replication and to ligate gene inserts into vectors during recombinant DNA procedures.
- Synthetase: General class of enzymes that catalyze the synthesis of molecules, often using energy from ATP.
- DNA Polymerase: Synthesizes new DNA strands by adding free nucleotides complementary to a template strand.
- Restriction Endonuclease: Functions as molecular "scissors" that cut DNA at specific nucleotide sequences.
Step 3: Final Answer
DNA ligase is the enzyme used to join the ends of two DNA strands.
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