Step 1: Understanding the Question:
This question tests knowledge of cloning vectors.
Step 2: Detailed Explanation:
Shuttle vectors can be used in two different hosts/systems.
They have origins of replication for both hosts (e.g., E. coli and yeast).
Cosmids (A): Hybrid of plasmid and phage lambda cos sites.
Phagemids (B): Hybrid of phage and plasmid.
Plasmids (D): Small circular DNA, used in bacteria.
Thus, shuttle vectors are used in two different systems.
Step 3: Final Answer:
Thus, shuttle vectors are used in two different systems, which corresponds to option (C).
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