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Question:
The source of Taq polymeraise used in $PCR$ is a
The source of Taq polymeraise used in $PCR$ is a
Updated On: Aug 1, 2022
- thermophilic fungus
- mesophilic fungus
- thermophilic bacterium
- halophilic bacterium
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The Correct Option is C
Solution and Explanation
Taq polymerase used in PCR is obtained from the thermophilic bacterium, Thermus aquaticus. This produces two double- stranded DNA molecules, which following their heat- denaturation, can serve as template for another cycle of amplification.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.
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