The recommended method for seed germination test in mustard is-
Show Hint
Small seeds (Mustard, Tobacco, Lucerne) $\rightarrow$ TP (Top of Paper).
Medium/Large seeds (Wheat, Maize, Pulses) $\rightarrow$ BP (Between Paper).
Very large/Hard seeds $\rightarrow$ Sand.
- Sand method
- Top of paper method
- Between paper method
- Sowing in field
The Correct Option is B
Solution and Explanation
The question asks for the standard laboratory method used to test the germination percentage of Mustard seeds ($Brassica$ spp.).
Standard protocols are defined by ISTA (International Seed Testing Association).
Step 2: Detailed Explanation:
• Germination Test Methods:
• Top of Paper (TP): Seeds are placed on top of one or more layers of moist filter paper or towel paper in a Petri dish or on trays.
• Between Paper (BP): Seeds are placed between two layers of moist paper (often used for larger seeds like maize).
• Sand (S): Seeds are sown in a layer of moist sand.
• Selection Criteria: The choice of method depends on the seed size and the light requirement for germination.
• Mustard Seeds: Mustard seeds are small and often require light to stimulate germination.
• The "Top of Paper" (TP) method is ideal because it allows light to reach the seeds and makes it easy to observe the tiny radicle and plumule emergence.
• According to ISTA, for $Brassica$ species, the prescribed temperature is usually $20-30^\circ C$ (alternating) or a constant $20^\circ C$ using the TP method.
Final Answer:
The "Top of Paper" (TP) method is the officially recommended method for conducting seed germination tests in Mustard.
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