Question

The pH of animal cell tissue culture is usually:

• A. pH 6.4
• B. pH 7.4
• C. pH 8.4
• D. pH 9.4

Hint:

Cell culture pH: 7.4 (physiological).
Buffers: HEPES, bicarbonate.
Phenol red is used as pH indicator.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
Animal cells require specific pH for growth.
The pH must be maintained for cell survival.

Step 2: Key Formula or Approach:
No specific formula.
Need to know the optimal pH for cell culture.

Step 3: Detailed Explanation:
Animal cell tissue culture is usually maintained at pH 7.4.
This is similar to physiological pH.
A buffer system (e.g., HEPES or bicarbonate) is used.
Thus, the correct pH is 7.4.

Step 4: Final Answer:
The pH of animal cell tissue culture is usually pH 7.4.
Hence, the correct option is (B).