During photorespiration, RuBisCo catalyzes oxygenation of RuBP (5-C) in the presence of O{2}. This reaction produces one molecule of 3-phosphoglycerate (3-C) and one molecule of 2-phosphoglycolate (2-C). 2-phosphoglycolate is further metabolized in peroxisomes and mitochondria, releasing CO{2}. This reduces photosynthetic efficiency.