Question:
The heart of HPLC is the
The heart of HPLC is the
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In HPLC, the column accomplishes the fundamental separation of analytes, making it the central component (heart) of the instrument.
Updated On: Sep 15, 2026
- Injector
- Column
- Solvent
- Defector
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The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept:
High-Performance Liquid Chromatography (HPLC) separates chemical analytes based on differential partitioning between mobile and stationary phases.
Step 2: Detailed Explanation:
The chromatographic analytical column contains the micro-particulate stationary phase (e.g., C18 silica particles, $3\text{--}5\,\mu\text{m}$).
It is where the actual differential molecular interactions, retention, resolution, and separation of chemical constituents take place under high pressure, earning it the universal designation as the 'heart of HPLC'.
Step 3: Final Answer:
Therefore, the column is the heart of HPLC.
High-Performance Liquid Chromatography (HPLC) separates chemical analytes based on differential partitioning between mobile and stationary phases.
Step 2: Detailed Explanation:
The chromatographic analytical column contains the micro-particulate stationary phase (e.g., C18 silica particles, $3\text{--}5\,\mu\text{m}$).
It is where the actual differential molecular interactions, retention, resolution, and separation of chemical constituents take place under high pressure, earning it the universal designation as the 'heart of HPLC'.
Step 3: Final Answer:
Therefore, the column is the heart of HPLC.
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