Question:

Staphylococcus pseudintermedius can be identified on the basis of following characters
A. Coagulase negative
B. Susceptible to polymyxin B
C. V P test positive
D. \(\beta\)- galactosidase positive
Choose the correct answer from the options given below:

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Differential Diagnostic Rule: S. pseudintermedius vs S. aureus:
S. pseudintermedius = Polymyxin B Susceptible, ONPG ($eta$-galactosidase) Positive, VP Negative.
S. aureus = Polymyxin B Resistant, ONPG Negative, VP Positive.
  • B and D only
  • A and C only
  • A and B only
  • C and D only
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The Correct Option is A

Solution and Explanation


Step 1: Understanding the Concept:

Phenotypic and biochemical differentiation of Staphylococcus intermedius group (SIG) members from Staphylococcus aureus in veterinary clinical microbiology.

Step 2: Detailed Explanation:

Diagnostic biochemical profile of Staphylococcus pseudintermedius (the major pyogenic pathogen in canine pyoderma and otitis):
1. Coagulase Test: It is Coagulase-positive (clots rabbit/canine plasma), ruling out character A.
2. Polymyxin B Susceptibility (B): It is characteristically susceptible to polymyxin B ($300\text{ units}$ disc), differentiating it from polymyxin B-resistant S. aureus.
3. Voges-Proskauer Test (C): It is VP-negative (acetoin negative), whereas S. aureus is VP-positive.
4. $\beta$-Galactosidase Activity (D): It is ONPG positive (possesses $\beta$-galactosidase), unlike S. aureus which is ONPG negative.
Therefore, characters B and D accurately identify S. pseudintermedius.

Step 3: Final Answer:

Thus, the correct answer is B and D only, corresponding to option (A).
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